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APPENDIX. THE SCIENCE OF PLANT BIOTECHNOLOGY
1. I cannot resist an example. Here are the crucial steps in construction of the vector used to insert the genes for beta-carotene synthesis into rice, as given in the paper cited as note 3: “Finally, crtI and psy expression cassettes were isolated with Kpn I/Not I digestion and inserted into Kpn I/Not I-digested pUC18M and designated as pBaal3. pBin19hpc was made by insertion of a Kpn I fragment